What is CCK 8 solution?

CCK-8 is a one-bottle solution; no premixing of components is required. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.

How does CCK 8 assay work?

Product overview. Cell Counting Kit 8 (WST-8 / CCK8) (ab228554) provides a convenient and robust way of performing a cell viability assay. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.

How does CCK promote satiety?

As a peptide hormone, CCK mediates satiety by acting on the CCK receptors distributed widely throughout the central nervous system. The mechanism for hunger suppression is thought to be a decrease in the rate of gastric emptying.

How do you interpret cell viability?

Cell viability can be calculated using the ratio of total live/total cells (live and dead). Staining also facilitates the visualization of overall cell morphology. NOTE: Trypan Blue has a greater affinity for serum proteins than for cellular protein.

How is CCK produced?

Cholecystokinin is produced by I-cells in the lining of the duodenum and is also released by some neurons in the brain. It acts on two types of receptors found throughout the gut and central nervous system. The most recognised functions of this hormone are in digestion and appetite.

What is CCK secretion?

Cholecystokinin (CCK) is a hormone secreted by the I-cells of the upper small intestine in response to fat, protein, and some nonnutrients, for example, camostat, and a peptide/neurotransmitter secreted by neurons of the central and peripheral nervous systems.

How to use CCK-8 reagent?

Cell Counting Kit-8 (CCK-8) is ready-to-use solution. Mix the reagent to ensure a homogenous solution before use. Pay attention to the edge effect of 96-well plate. It is suggested to discard the surrounding plate hole and add the same amount of PBS.

What is CCK-8 used for?

Cell Counting Kit-8 (CCK-8) provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt.

Is CCK-8 toxic to cells?

Is CCK-8 toxic to cells? Since the toxicity of CCK-8 is so low, the same cells can be used for other cell proliferation assays such as the crystal violet assay, neutral red assay or DNA fluorometric assay after the CCK-8 assay is completed. Q. How stable is CCK-8?

Is CCK-8 more sensitive than other tetrazolium salts?

The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1. 1. 100μL cell suspension was inoculated on 96-well plate and incubated in cell incubator (37°C, 5% CO2).