How does TMV replicate?
Abstract. The replication of tobacco mosaic virus (TMV) RNA involves synthesis of a negative-strand RNA using the genomic positive-strand RNA as a template, followed by the synthesis of positive-strand RNA on the negative-strand RNA templates.
Which insect is vector of TMV?
First, pollinators such as bumble bees used in the pollination of some greenhouse crops, like cucumbers and tomatoes, can spread TMV. Also, larger chewing insects – not common in greenhouse production – such as grasshoppers can spread TMV.
How many Capsomeres are present in TMV?
TMV is a simple rod-shaped helical virus consisting of single-stranded RNA enveloped by a protein coat called capsid. This capsid is made of 2,130 sub units called capsomeres. There are 49 capsomeres on every three turns of the helix.
Is tobacco mosaic virus a retrovirus?
Tobacco mosaic virus (TMV) is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family Solanaceae….
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What is TMV describe its structure and reproduction?
Structure of Tobacco Mosaic Virus (TMV): TMV is a simple rod-shaped helical virus (Fig. 13.20) consisting of centrally located single- stranded RNA (5.6%) enveloped by a protein coat (94.4%). The rod is considered to be 3,000 Å in length and about 180 Å in diameter. The protein coat is technically called ‘capsid’. R.
What is TMV and structure?
Tobacco mosaic virus (TMV; Tobamovirus, Virgaviridae) is a rodlike virus with a length of 300 nm and diameter of 18 nm. TMV capsids are composed of 2130 identical protein subunits, which assemble around the viral ssRNA to form a helical structure, with a hollow central cavity of 4 nm diameter.
What pathogen causes TMV?
The tobacco mosaic virus (TMV) infects tobacco plants and other closely related species, such as tomatoes and peppers. TMV is transmitted by contact between plants, either naturally or on the hands of farmers or contaminated clothes or tools.
Is TMV spread by aphids?
It is well known that many plant viruses can be transmitted by aphids, but the highly infectious tobacco mosaic virus (TMV) seems to have no aphid vector.
What is the significance of capsomeres in TMV?
– Numbers of capsomeres in TMV are 2130 because on every three turns of the helix, 49 capsomeres are present on every three turns of the helix. Hence, the correct answer is option (B).
What are capsomeres made of?
The penton capsomere is composed of a homotrimeric fiber and homopentameric penton base at the 12 vertices of the icosahedron. Together with fiber, penton base has a major role in Ad cellular internalization.
Does TMV use reverse transcriptase?
Tobacco mosaic virus (TMV) can cause a severe disease that is capable of greatly reducing tobacco quality and yield. In this study, a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of TMV.
What is the composition of TMV?
Which is the popular TMV experiment?
In an experiment, common Tobacco Mosaic Virus (TMV) and its mutant strain ‘HR’ were used to prepare hybrid particles with ‘HR’ nucleic acid and ‘TMV’ protein coat. These hybrids were mixed with antibodies against ‘HR’ strains.
What is the genetic material of TMV?
The genetic material in tobacco mosaic virus is RNA.
What is TMV in plants?
Tobacco mosaic virus (TMV) chlorosis seen in a backlit tobacco leaf (Nicotiana tabacum) The tobacco mosaic virus infects tobacco and lots of other closely related species like tomatoes and peppers. It is transmitted by contact between plants, either naturally or on the hands of farmers.
How TMV is transmitted and control?
TMV is very easily transmitted when an infected leaf rubs against a leaf of a healthy plant, by contaminated tools, and occasionally by workers whose hands become contaminated with TMV after smoking cigarettes. A wounded plant cell provides a site of entry for TMV.
What does TMV do to plants?
TMV infects the chloroplasts of plant leaves and reduces the plant’s ability to photosynthesise . The leaves change colour from green to yellow or white and develop a mosaic pattern. It can also make leaves crinkled or curled up.
What causes TMV in plants?
Transmission from plant to plant TMV is very easily transmitted when an infected leaf rubs against a leaf of a healthy plant, by contaminated tools, and occasionally by workers whose hands become contaminated with TMV after smoking cigarettes. A wounded plant cell provides a site of entry for TMV.