Which stain is used for histochemical demonstration of lipids?
Histochemical demonstration of Lipids – Uses/Application The lipid accumulation can be observed by staining it with Sudan black or Osmium tetroxide.
What is histochemical staining?
A staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids, and mucins in tissues and fungal hyphae.
Which staining is best for lipid detection?
The Oil Red O (ORO) stain can identify neutral lipids and fatty acids in smears and tissues. Fresh smears or cryostat sections of tissue are necessary because fixatives containing alcohols, or routine tissue processing with clearing, will remove lipids. The ORO is a rapid and simple stain.
What is Sudan black stain?
Black Sudan B is used for the staining of a wide variety of lipids such as phospholipids, steroles and neutral triglycerides. Black Sudan B is not lipid specific like other Sudan dyes and can also be used for chromosome staining, Golgi apparatus and leukocyte granules.
What is histochemical reaction?
Histochemistry refers to procedures in which tissue sections act as the medium in which biochemical reactions are carried out by the addition of substrates, inhibitors, or other chemicals.
What are examples of special stains?
- Massons Trichrome. The trichrome stain helps to highlight the supporting collagenous stroma in sections from a variety of organs.
- Verhoff’s Elastic Stain.
- Reticulin Stain.
- Giemsa Stain.
Is an example of special staining?
Among these are the giemsa stain and the Wright’s stain (or Wright-Giemsa stain). The latter is utilized to stain peripheral blood smears. The giemsa stain can be helpful for identifying components in a variety of tissues. One property of methylene blue and toluidine blue dyes is metachromasia.
Why do medical scientists apply histochemical stains to samples of tissues and cells?
Staining is used to highlight important features of the tissue as well as to enhance the tissue contrast. Hematoxylin is a basic dye that is commonly used in this process and stains the nuclei giving it a bluish color while eosin (another stain dye used in histology) stains the cell’s nucleus giving it a pinkish stain.
Which is the optimal temperature for a histochemical reaction?
Most unfixed material will section well between −15 and −23°C. Tissues containing large amounts of water will section best at the warmer temperature, and harder tissues and those which contain fat require a colder temperature.
What is Prussian blue stain used for?
Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.
How do you explain the histophysical and histochemical reactions of lipids?
Thus, the histophysical and histochemical reactions of lipids can in part be explained by consideration of these surface (water-lipid phase) phenomena. Among histophysical methods, lipid-soluble dyes, birefringence, and extraction techniques are commonly employed.
What is the purpose of lipid staining?
Lipid staining is a useful technique that is used for demonstrating intracellular lipids in various tissue sections.
What stains do we offer for Histochemistry?
We offer a variety of routine and special histochemicals stains that may be performed on paraffin embedded tissue sections or frozen tissue sections to visualize specific elements of interest. Our scientists are highly experienced in the optimization and application of special stains for selective staining of unique tissue elements of interest.
Which histophysical methods are used to extract lipids?
Among histophysical methods, lipid-soluble dyes, birefringence, and extraction techniques are commonly employed. In the latter category, the extraction of free lipids with chloroform-methanol and the differential extraction of hydrophobic lipids with acetone appear to be reliable.